The objective of this study was to evaluate the resting postures of the fingers and wrist based on the biomechanical model in term of hand posture (neutral, pronation, and supination) and gender (male and female). The finger and wrist joint angles were measured with VICON motion system. The EMG system was used to examine the muscle activity in the resting condition. The participants consisted of twenty male and twenty female students. The angles of the fingers and wrist were analyzed by means of the coordinate system associated with the International Society of Biomechanics. Hand posture was significant for all the joints. The finger and wrist joint flexed in supination more than in neutral and pronation. The hand posture and gender were not significant for the results of muscle activity, but it had larger muscle activities in supination more than in neutral and pronation.