Biomachining using microorganisms to remove metal from a workpiece is an alternative technology for machining processes that may have environmental advantages. Studies of the characteristics of micromachining using microorganisms such as Acidithiobacillus ferrooxidans will explore and improve the capabilities of this alternative machining process. The objective of this study is to investigate the surface roughness and the material removal rate characteristics in biomachining of copper for various machining times. We present the biomachining of copper using A. ferrooxidans, including bacterial culturing and workpiece preparation, and a comparison of the surface roughness, visible surface appearance of the workpiece, and material removal rate before and after biomachining process.