Light emitting diode(LED) technology has been increasingly developed and larger color gamut by LED illuminations can be reproduced; therefore more efficient LED lighting design can be accomplished under a consideration of color emotion. Fifty-two LED colors which are uniformly distributed on the uniform chromaticity space are evaluated in terms of fatigueness and preference and their relation to three color-appearance attributes(lightness, chroma and hue) are investigated. As a result, 23 human observers likely to prefer and feel comfortable, when lightness of a given LED color stimulus increases as well as its chroma decreases. The highest fatigueness score is observed in red color series and the most preferred LED color is found in green color series. In addition, fatigueness and preference show a strong negative linear relation and their Pearson correlation is higher than -0.8.