This study performs the subjective evaluations of LED illumination conditions according to three types of color temperatures (7,000[K], 5,000[K], 3,000[K]) after employing LED illumination system in the classroom, Since the objective of this study is to develop an artificial lighting conditions like day light comfortable to students in the classroom, the learning effect based on three types of LED illumination conditions are analyzed. Three factors (learning intention, learning environment, learning motivation) are extracted by ANOVA and there are preference differences of LED illumination conditions between learning intention and learning environment factors. Especially, preference differences of LED illumination conditions are existed about calculation, reading and fatigue reduction. The test results of this study can be applied to obtaining high achievement of learning based on the lighting conditions.