This study deals with the performance evaluation on waterproofing and root penetration resistance membrane which is made from combining both the fiberglass reinforced plastic and the self adhesion butyl rubber sheet for Green Roof System. The key to constant development and resupply/replenishment of Green Roof for the stability of green roof system is the proper installation and the long-term preservation of the waterproofing and root penetration resistance membranes. Problems with the waterproofing and root penetration resistance membranes undermine the safety of the concrete slabs, which leads to leakage incidents. It is therefore important to make waterproofing and root penetration resistance membranes which can completely block the root penetration. This research showed that the FRP root penetration resistance layer is effective in blocking the intrusion of the plant roots, and that the flexibility of the self adhesion butyl rubber sheet makes it easy to withstand against damage from the movement of the concrete substrate. As follows, when using the waterproofing and root penetration resistance membrane which combines the two layers, long-term stability of the structures and the further development of the Green Roof industry are expected.