Yeonguemjiri-tang(連芩止痢湯, YGT) exhibits potent anti-inflammatory activity in widely intestine disease, but its mechanism undisclosed. To elucidate the molecular mechanisms of YGT on pharmacological and biochemical actions in inflammation, we examined the effect of YGT on pro-inflammatory mediators in phorbol 12-myristate 13-acetate (PMA) plus A23187-induced mast cell and lipopolysaccharide (LPS)-stimulated macrophages. The investigation focused on whether YGT inhibited pro-inflammatory cytokines such as interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-${alpha}$ (TNF-${alpha}$) in PMA plus A23187-induced HMC-1 cells and inflammatory madiators such as nitric oxide (NO), TNF-${alpha}$, IL-6, iNOS, COX-2 in LPS-stimulated RAW 264.7 cells. We found that YGT inhibited LPS-induced NO, TNF-${alpha}$ and IL-6 productions as well as the expressions of iNOS and COX-2. These results suggest that YGT has inhibitory effects on mast cell-mediated and macrophage-mediated inflammation.