This paper describes experimental study on GM-type pulse tube refrigerator with neon as a working fluid instead of helium. Neon gas has similar compression characteristics with helium gas because it is a monoatoimc gas. In experiments, a cooling performance test was performed with same compressor and pulse tube refrigerator for neon and helium as working gas. From experimental results, a PTR with neon show the improved cooling performance than a PTR with helium. Cooling performance and operating characteristics of a PTR were discussed and compared for two different working gas.