This paper describes experimental investigation on neon refrigeration system using commercial helium compressor. In this paper, neon refrigeration cycle is calculated with assumption of ideal heat exchanger. From analysis, 32.6 K of the lowest temperature and 0.945 of quality after expansion are predicted. Cryogenic heat exchangers for pre cooler and main heat exchanger are designed and fabricated with configuration of tube-in-tube heat exchanger. In experiments, cooling performance test are performed as variation of charging pressure and orifice hole diameter. From experimental results, the lowest temperature of 44.0 K was measured with 500 ${mu}m$ orifice and 1500 kPa of charging pressure.