Characteristics of carburized surface layers in 0.18C-3.5Ni-1.5Cr-0.2Mo steels for main shaft bearings of wind turbines have been analyzed and evaluated before and after rolling contact fatigue tests. Mixed microstructure consisting of retained austenite and tempered martensite has been formed with compressive residual stresses in the surface hardened layers of the specimens showing uniform hardness distribution with value about Hv700 after vacuum carburizing and tempering. It has been found on the raceway of the layers of the specimens after rolling contact fatigue tests that the amount of retained austenite decreased and compressive residual stresses increased, resulting from cyclic contact stresses applied during the tests. It has been also revealed that higher durability of the bearings can be obtained through controlling the amount of the retained austenite in the surface of the bearing steels to be lower in this study.