The Nemopilema nomurai is a large jellyfish attaining a weight of 200 kg and bell diameter of 2 m when fully grown. To prevent damage to this species, this study determined the acoustic characteristics of N. nomurai using frequencies 38 and 120 kHz. The CPUE of N. nomurai and the averaged SV of 38 and 120 kHz had a lower (+) correlation coefficient and relationship at 120 kHz (R=0.51) than at 38 kHz (R=0.15) was significant. In addition, the averaged SV at 120 kHz was higher than at 38 kHz. The ${Delta}MVBS_{120-38}$ in section catches ${geq}97.8%$ wet mass of N. nomurai was -2.2 to 5.6 dB. The ${Delta}TS_{120-38}$ in situ TS was extracted in sections catches of only N. nomurai by FMT. It was found that the averaged in situ ${Delta}TS_{120-38}$ were at 0.6 and 0.1 dB. Furthermore, it results showed a close relationship between the bell diameter and TS of N. nomurai. The dominance of smaller N. nomurai (11.0~20.0 cm bell diameter in the air) corresponded to a similar proportion of low TS values (-69.0~-65.0 dB). A small number of larger N. nomurai (25.0~38.0 cm bell diameter in the air) were collected, in which TS values were the highest (-62.0~-58.0 dB).