This paper explores the influence of oxidation time on selective oxidation of regenerated cellulose with $NO_2/CCl_4$ as oxidation system. FTIR and other testing methods are used to characterize the structure of oxidized regenerated cellulose (ORC), showing that the regenerated cellulose is oxidized at C6 by the $NO_2/CCl_4$ system with extremely high selectivity. With the extension of oxidation duration, the carboxyl content in ORC increases gradually from 12.11 to 21.29 % while the degree of polymerization (DP) decreases from 92.86 to 53.40 and the strength of monofilament decreases gradually. In case of the reaction duration of 24 h, the performance of oxidation product can satisfy the requirement of absorbing hemostatic materials, i.e., with good hemostatic performance, while it is also provided with excellent antibacterial activity, which the reduction in colony forming units (CFU) is more than 99.9 %.