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Alginate Hydrogel Embedding Poly(D,L-lactide-co-glycolide) Porous Scaffold Disks for Cartilage Tissue Engineering
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  • Alginate Hydrogel Embedding Poly(D,L-lactide-co-glycolide) Porous Scaffold Disks for Cartilage Tissue Engineering
  • Alginate Hydrogel Embedding Poly(D,L-lactide-co-glycolide) Porous Scaffold Disks for Cartilage Tissue Engineering
저자명
Choi. Sung-Wook,Moon. Seung-Kwan,Chu. Ji-Yeon,Lee. Hye-Won,Park. Tae-Joon,Kim. Jung-Hyun
간행물명
Macromolecular research
권/호정보
2012년|20권 5호|pp.447-452 (6 pages)
발행정보
한국고분자학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Small poly(D,L-lactide-co-glycolide) scaffold disks (2 mm in diameter) with a bimodal porous structure of $237.5{pm}34.2{mu}m$ for large pores and $9.2{pm}3.9{mu}m$ for micropores were prepared by gas-forming and controlled-precipitation methods. Chondrocyte-seeded scaffold disks were dispersed in an alginate solution containing culture media. The dispersion was transferred into a Teflon mold with a specific shape, eventually forming an alginate hydrogel (approximately 1 cm in dimension) that contains cell/disk aggregates after crosslinking using $CaCl_2$. In vitro assessment of the alginate hydrogel showed significant increases in DNA and glycosaminoglycan content after 21 days, suggesting that chondrocyte still proliferated on the small scaffold disks in the alginate hydrogel despite its large dimensions. Moreover, in vivo immunohistochemical analysis of the alginate hydrogel demonstrated that chondrocytes secreted native extracellular matrix proteins (e.g., collagen, proteoglycan). We believe that our approach of using alginate hydrogel containing cell/disk aggregates with a shape fitted to the target site could provide a versatile platform to engineer large and thick tissues and/or organs.