In this paper, we study on how to determine the number of hidden layer neurons in neural network for predicting defect size of steam generator tube. It was reported in the literature that the number of hidden layer neurons can be efficiently determined with the help of cross-validation. Although the cross-validation provides decent estimation performance in most cases, the performance depends on the selection of validation set and rather poor performance may be led to in some cases. In order to avoid such a problem, we propose to use multifold cross-validation. Through the simulation study, it is shown that the estimation performance of defect width (defect depth, respectively) attains 94% (99.4%, respectively) of the best performance achievable among the considered neuron numbers.