The transmission of the world Installed in an Office occurs through the air. Preventing that transmission, especially in indoor environments like those in airplanes, schools and offices, is a major public health concern. in this study the real situation of lab which is consist of dilution, Ultraviolet-C based air sterilization system. This model include indoor microbe transport which is generated human source. a computer simulation was performed to determine if such simulation can be used effectively to predict the more accurate, but difficult to perform, actual physical experiment. Result show that CFD can be modeled microbe transport effectively and it can be visualized microbe transport separation from flow streamline. also it make a good prediction of microbe transport.