The aims of this study were three. First, to examine the degree of deviation from dictionary prescribed symbols and actual speech made by American English speakers. Second, to measure the frequency of vowel and consonant production of American English speakers. And third, to investigate gender differences in the segmental sounds in a speech corpus. The Buckeye Speech Corpus was recorded by forty American male and female subjects for one hour per subject. The vowels and consonants in both the phonemic and phonetic transcriptions were extracted from the original files of the corpus and their frequencies were obtained using codes of a free software R. Results were as follows: Firstly, the American English speakers produced a reduced number of vowels and consonants in daily conversation. The reduction rate from the dictionary transcriptions to the actual transcriptions was around 38.2%. Secondly, the American English speakers used more front high and back low vowels while three-fourths of the consonants accounted for stops, fricatives, and nasals. This indicates that the segmental inventory has nonlinear frequency distribution in the speech corpus. Thirdly, the two gender groups produced vowels and consonants similarly even though there were a few noticeable differences in their speech. From these results we propose that English teachers consider pronunciation education reflecting the actual speech sounds and that linguists find a way to establish unmarked segmentals from speech corpora.