The aim of the present study was to evaluate dietary effects of dietary nucleotides (NTs) on developmental parameters in second-generation Sprague-Dawley rats. Experimental design was set up as six groups, including one control and five nucleotide administered groups, doses ranged from 0.01% to 1.28% nucleotides. First-generation (F0) rats were divided into two terms: (1) fed for 90-day study; (2) mated for offspring (F1). After weaning, each group of second-generation F1 rats was fed the control diet. Evaluating parameters were weight gain and food utilization ratios of rats, hematological and biochemical parameters, and organ pathology. The results showed that there was a greater weight gain and food utilization ratio in weaned rats (F0 and F1). Furthermore, weight gain and food utilization ratios were increased in the first 2 weeks of F1 male and the first week of F1 female rats. There were no significant changes in blood indicators of NT groups with the exception of decrease in aspartate aminotransferase and serum uric acid levels. Overall, it was demonstrated that NT supplements could promote the early growth and development at a 0.01% dose. Although NTs may have cumulative reproductive effects, they were safe even at a high-dose supplementation. Additionally, NT supplementation could promote restoration and optimize liver function.