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Evaluation of In Vitro Antioxidant and In Vivo Analgesic Potential of Terminalia paniculata Aqueous Bark Extract
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  • Evaluation of In Vitro Antioxidant and In Vivo Analgesic Potential of Terminalia paniculata Aqueous Bark Extract
  • Evaluation of In Vitro Antioxidant and In Vivo Analgesic Potential of Terminalia paniculata Aqueous Bark Extract
저자명
Talwar. Sahil,Nayak. Pawan G.,Mudgal. Jayesh,Paul. Piya,Bansal. Punit,Nandakumar. Krishnadas
간행물명
Journal of medicinal food
권/호정보
2013년|16권 12호|pp.1153-1161 (9 pages)
발행정보
한국식품영양과학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The Terminalia genus includes plants that are used in a variety of food, nutritional products, and traditional medicines. Aqueous bark extract of Terminalia paniculata (TPW) was screened for its antioxidant and analgesic potential. The major polyphenols were identified by high-performance liquid chromatography. In vitro antioxidant potential of TPW was investigated by 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) ($ABTS^{2-}$) radical assay, nitric oxide (NO) scavenging, superoxide scavenging ($O^{2-}$), $Fe^{2+}$ chelating (O-phenanthroline), and ferric reducing/antioxidant power (FRAP) assay. We evaluated the effects of TPW on cell viability, lipopolysaccharide (LPS)-stimulated reactive oxygen species (ROS), nitrite, and cytokines (interleukin [IL] 6 and tumor necrosis factor alpha [TNF-${alpha}$]) in RAW264.7 murine macrophages. Evaluation of analgesic activity of TPW was performed using acetic acid-induced writhing and hot plate test in mice. Phytochemical analysis showed the presence of four polyphenols, namely, gallic acid, ellagic acid, rutin, and quercetin. TPW showed maximum superoxide, $ABTS{2-}$, NO, DPPH inhibition, and $Fe^{2+-}$chelating property at $400{mu}g/mL$, respectively. FRAP value was 4.5-0.25 ${mu}g$ Fe(II)/g. TPW, per se, did not affect RAW264.7 cell viability. In LPS-induced RAW 264.7 cells, TPW attenuated the elevation in ROS, nitrite, IL-6, and TNF-${alpha}$ levels. TPW (100-400mg/kg, orally) significantly reduced the number of writhes in a dose-dependent manner compared with the control. Similarly, TPW (400mg/kg, orally) evoked a significant increase in the maximum percentage effect in the hot plate test. The study suggests the efficacy of aqueous bark extract of T. paniculata as a potential antioxidant and analgesic agent.