Purpose: The purpose of this study was to compare professional self-concept, clinical nursing competence, and practicum satisfaction between nursing students with and without pediatric nursing clinical preceptorship. Methods: This study used a comparative research design. A total of 85 junior nursing students participated in the study, and data from 76 students were analyzed using SPSS WIN 20.0. Among them, 44 students experienced pediatric nursing clinical preceptorship. Results: The students with clinical preceptorship showed a significantly higher score in nursing skill related items of professional self-concept and the confidence in knowledge of injection preparation and administration compared to the counterpart students. Practicum satisfaction was also significantly higher in the students with clinical preceptorship than in the other students. There were significant positive correlations among professional self-concept, performance confidence, and practicum satisfaction. Conclusion: The study findings suggest that clinical preceptorship is an effective educational method to teach clinical practicum of pediatric nursing. The use of clinical preceptorship needs to be considered to motivate nursing students actively involved into the clinical practicum. The roles of clinical preceptors should be clearly identified in clinical education.