Purpose: The purpose of the study was to examine associations between job stress and work-related musculoskeletal symptoms (WRMS) in street sanitation workers. Methods: A cross-sectional correlational study was conducted with 87 male street sanitation workers at E Gu in Seoul. The Job stress and WRMS were measured by using the Korean Occupational Stress Scale-Short Form and the KOSHA GUIDE H-9-2012 instrument, respectively. The WRMS was evaluated according to the National Institute of Occupational Safety and Health criteria. Results: The participants reported greater levels of insufficient job control and interpersonal conflict than the general Korean population. Of the participants, 44.8% reported WRMS at any body parts. Specifically, 28.7% reported WRMS at upper parts, while 16.5% reported WRMS at lower parts. Compared to low levels of job demand, its high levels were significantly associated with WRMS at both upper and lower parts of the body (OR=3.62, 95% CI=1.15~11.38; OR=3.60, 95% CI=1.04~12.39). Compared to low levels of occupational climate, its high levels were significantly associated with WRMS at upper parts of the body (OR=3.18, 95% CI=1.22~8.26). Conclusion: Among street sanitation workers, job stress may be a correlate of WRMS. Therefore, nursing strategies for reducing job stress are needed to prevent and manage WRMS.