The purpose of this study was to investigate the effects of knee joint muscle fatigue and overweight on the angular displacement and moments of the lower limb joints during landing. Written informed consent forms, which were approved by the human subject research and review committee at Dong-A University, were provided to all subjects. The subjects who participated in this study were divided into 2 groups: a normal weight group and an overweight group, consisting of 15 young women each. The knee joint muscle fatigue during landing was found to increase the dynamic stability by minimizing the movements of the coronal and horizontal planes and maintaining a more neutral position to protect the knee. The effect of body weight during landing was better in the normal weight group than in the overweight group, with the lower limbs performing their shock-absorbing function in an efficient manner through increased sagittal movement. Therefore, accumulated fatigue of knee joint muscles or overweight may be highly correlated with the increase in the incidence of injury during landing after jumping, descending stairs, and downhill walking.