Objectives : The purpose of the study is to investigate the relationship between humor and empowerment in clinical dental hygienist. Methods : Subjects were 374 clinical dental hygienist in Jeollabuk-do from April 20 to June 4, 2013. Data were analyzed using the statistical package SPSS WIN 12.0 for frequency, mean and standard deviation analysis, one-way ANOVA and linear regression. Results : The mean score or the clinical dental hygienist of humor was 2.55 out of 4 each of the sub-dimensions including emotional expressiveness, message sensitivity and preference of humor. Empowerment was 3.50 out of 5 and each of the sub-dimensions including meaning, competence, self-determination and impact. There was a significantly positive correlation between the sense of humor and the empowerment. Conclusions : It is necessary to develop the empowerment combined with humor sense in the curricula of dental hygiene education and leadership management in the clinical dental hygienist.