This study was performed to reveal the relationships between various gas supply conditions including inlet numbers and positions for Photobioreactor. To do that, this study was installed the experimental apparatus. All experiments were performed for the cases with 1, 2, 3, and 4 inlets and for gas flow rate of 4~8 lpm. Through the experiments, this study showed that the case with 3 or 4 inlets could reduce about 50% of the pressure loss head for all gas path than that of one inlet base case. So, these results can be used as basic data to design the gross or multiple photobioreactor.