Objectives: The purpose of this study was to identify the effects of job characteristics on smoking, and the mediating effects of job stress between job characteristics and smoking among elderly workers. Methods: This study employed data from the Korean Longitudinal Study of Ageing, and a total of 778 older workers were used for analysis. Current smoking status, job stress, job characteristics including physical job demands, job demands related to contents, working days, regularity of working hour, satisfaction with income, and sociodemographic factors were analyzed. Descriptive analysis, t-tests, chi-square test, multiple linear regression, and logistic regression were used for analysis. Results: Results showed that physical job demands and regularity of working hours were significant predictors of smoking of elderly workers. The higher level of physical job demands and regular working-hour increased the risk of smoking. Also, physical job demands, job demands related job contents, and income satisfaction directly impacted on job stress. However, job stress did not mediate the relationship between job characteristics and smoking. Conclusions: The findings suggest that smoking interventions for older workers should take into account job characteristics. Implications for practice and future study directions are discussed.