The purpose of this study was to examine nurses' knowledge level, attitudes, and preventive practice of pressure ulcers in hospitalized patients. Methods: For this descriptive study, 226 nurses from one acute university hospital were selected. The data were collected using questionnaires from June to July, 2014. Analysis was done by t-test, ANOVA and Pearson correlation coefficient with SPSS/Win 21.0. Results: Levels of knowledge and visual differentiation ability of pressure ulcer were average 65.2 and 75.3 out of 100, and levels of attitudes, and preventive practice were average 3.70 out of 5 and 2.71 out of 3 respectively. There was significant difference in nurses' knowledge of pressure ulcer by experience of wound care education(F=7.888, p<.001), frequency of pressure ulcer care(F=5.608, p=.001), and area of practice(F=3.344, p=.006). The attitudes toward pressure ulcer prevention were positive correlated to preventive practice among nurses(r=.401, p<.001). Conclusion: Nurses attitudes toward preventive practice of pressure ulcer is important. And also the structured education about pressure ulcer assessment is needed.