Stress concentrations around discontinuities, such as a hole or a sudden change in cross section of a structural member, have great important cause in the most materials failure because the stress near the points of application of concentrated loads can reach values much larger than the average value of the stress in the member. This paper presents the stress concentrations between fillet and hole at different locations in a stepped plate under tensile loading. The analysis for interaction effect of stress concentration Analysis of Stress Concentration between Fillet and Hole in a Stepped Plate under Tensile Load by Photoelasticity was performed by photoelasticity and ANSYS which is a commercial finite element software. From the analysis results, the circular hole located at the different position from the fillet radius can cause different values of stress concentration factor within interacting region.