The purpose of this study was to illustrate what were important factors between curriculum and teaching-learning in the practical knowledge of pre-service special teachers which means university students who were studying in the field of special education. The questionnaire was divided into two parts, one was 12 curriculum questions and the other one was 12 teaching-learning questions. Total of 328 students were participated for this study. The one-way ANOVA and structural equation model were utilized for this study. The results were that: (a) the third grade was important time of years to develop the curriculum knowledge in practical knowledge, however the levels of school was not related with the curriculum knowledge; (b) the teaching-learning knowledge in practical knowledge was influenced by the grades and levels of school, and the teaching-learning adoption was also related by the grades and levels of school; and (c) the curriculum knowledge, the teaching-learning knowledge, and teaching-learning adoption had a effective relation to develop the practical knowledge. This study was concluded that the pre-service special teachers had a structure model in practical knowledge for teaching the students with disabilities.