The purpose of this study are to determine items and to establish criterion of health-ralated fitness for the college student of education, the prep teacher. The subjects of this study are consisted of both 99 male and 231 female on the 20-21 years old in sophomore of D. National Univ. of Education. For the factor analysis, heath-related fitness are measured height, body weight , BMI % fat( Inbody 3.0) simple harvard step test for PFI (physical fitness index) , grip strenth, sit up (1 min) standing long jump side step sitting trunk flexion eye-closed one foot balance (TAKEI made in Japan). It is used t-test to analyze sex difference. The ratation of factor axis through Normal Varimax Criterion is executed in order to yield the simple structure of factors each other male and remale. Test battery is made from factor analysis and evaluation criterion from ASCM\'s 10 step % is developed . To estimate % fat from health- related items is used multi-regression equation. The results of this study are summarized as floows\': 1. It is male\'s higher than female\'s health-related items, except % fat, sitting trunk flexion. 2. Helth-ralated fitness test battery of male is sit up, sitting trunk flexion, % fat and PFI, and female\'s % fat, eye-closed one foot balance, sitting trunk flexion, and PFI . 3. The test battery items each other male and female are eatablished evaluation criterion from ASCM\'s 10 step %. 4. Regression equation for % fat is included (+) BMI and (-) grip strength in male. (=) BMI, (-) standing long jump and (-) grip strength in female.