골 성장 및 개조에 관여하는 전신적 조절인자인 1,25-(OH)2D3와 국소적 조절인자인 TGF-β가 사람의 치주인대세포 기능에 미치는 영향을 관찰하고자 그 인자들을 단독 혹은 복합적으로 치주인대세포에 가하여 그 활성 변화를 측정 하여 다음과 같은 결과를 얻었다. 1. 10ng/ml 농도의 vitamin D3를 치주인대세포에 가한 후 배양 1, 2, 3 일째의 활성은 대조군과 차이가 없었으나, 50ng/ml 농도로 가한 후 배양 3일째에는 대조군에 비해 유의하게 증가하였으며, 100ng/ml 농도에서는 배양 1, 2, 3일째에 유의하게 증가하였다. 2. 0.1ng/ml 농도의 TGF-β를 치주인대세포에 가한 후 배양 1, 2, 3일째의 활성은 대조군과 유의한 차이가 없었으나, 1ng/ml나 5ng/ml 농도의 TGF-β를 가한 경우 배양 3일째 유의하게 증가되었으며 10ng/ml 농도의 경우에는 배양 2, 3일째에 유의하게 증가하였다. 3. 1ng/ml 농도의 TGF-β와 다양한 농도의 vitamin D3를 혼합투여한 경우, 100ng/ml 농도의 vitamin D3로 배양 3일째에 유의한 활성 증가를 볼 수 있었다. 4. 5ng/ml 농도의 TGF-β와 다양한 농도의 vitamin D3를 혼합투여한 경우, 10, 50, 100ng/ml의 vitamin D3에서 공히배양 2일째부터 유의한 활성 증가를 보였으나 10ng/ml에서는 배양 3일째에 그 활성이 유지되지 못하였다. 5. 10ng/ml 농도의 TGF-β와 다양한 농도의 vitamin D3를 혼합투여한 경우, 50ng/ml의 vitamin D3에서는 배양 2일째부터 100ng/ml의 vitamin D3에서는 1일째부터 유의한 활성 증가를 보였다.
TGF- β is a polypeptide with multiple physiological functions in regulation of cell-to-cell interaction and in growth and development. The active form of vitamin D3, 1,25-dihydroxycholecalciferol [1.25-(OH)2D3], is one of the most potent stimulators of osteoclastic acitivity. The purpose of this study was to evaluate the effect of Vitamin D3 and/or TGF- β on the periodontal ligament(PDL) cells. Human PDL cells were prepared from the first premolars extracted for the orthodontic treatment and were incubated in the environment of 37°C, 5% CO2 and 95% humidity. 10, 50 or 100ng/ml of 1,25-(OH)2D3. and 0.1, 1, 5 or 10ng/ml of TGF- β were administered to the culture wells, separately or in combination. And the viability of PDL cells was evaluated by MTT assay. The obtained results were as follows. 1. The viability of PDL cells in 10ng/ml of vitamin D3 was not significantly differenent from that of the control group at 1, 2 and 3-day of cultivation, but it was significantly increased in 50ng/ml of vitamin D3 at 3-day and in 100ng/ml of vitamin D3 at 2 and 3-day. 2. The viability of PDL cells in 0.1ng/ml of TGF- β was not significantly differenent from that of the control group at 1, 2 and 3-day of cultivation, but it was significantly increased in 1 and 5ng/ml of TGF-β at 3-day of cultivation, and in l0ng/ml of TGF- β at 2 and 3-day of cultivation. 3. In case of admixture of lng/ml of TGF-β and the various concentrations of vitamin D3, the viability of PDL cells was significantly increased in the admixture of 100ng/ml of vitamin D3 at 3-day of cultivation 4. In case of admixture of 5ng/ml of TGF-β and the various concentrations of vitamin D3, the viability of PDL cells began to be increased from 2-day of cultivation in the admixture of 10 50 and 100ng/ml of vitamin D3, but it was not maintained at 3-day in the admixture of 10ng/ml of vitamin D3 5. In case of admixture of 10ng/ml of TGF-β and the various concentrations of vitamin D3, the viability of PDL cells was significantly increased in the admixture of 50ng/ml of vitamin D3 at 2 and 3-day of cultivation, and in the admixture of 100ng/ml at 1, 2 and 3-day.
