It has been suggested that the frictional force between bracket and arch wire may impede the tooth movement. The present study was aimed to compare and analyze the effect of wire size, type of ligation, and duration of ligation on the magnitude of frictional force between cobalt chromium wire and stainless steel bracket under the artificial saliva. The results were as follows: 1. Type of ligation and size of wire were the main influencing factor on the level of friction. 2. Stainless steel ligature generated higher frictional forces thanelastomeric module. 3. The rectangular wire consistently exhibited more frictional force values than round wires, while there was no significant difference between frictional forces of round wires. 4. In elastic ligature, frictional force decreased with time. 5. Artificial saliva had no significant influence on the frictional force between cobalt chromium wire and bracket.