Whenever subway users use a subway, a typical means of transportation of modern society, they are\nalways exposed to the physical, mental resistance by the internal and external factors of the various attachments. Although the improvement in various traffic policies has been made for increasing the user satisfaction, it is an important task that for effectiveness, users receive less negative resistance which they feel. However, most of existing studies have been conducted with the qualitative method based on the recognition for user satisfaction evaluation. The research with this qualitative method has a limit in grasping objectively and quantitatively, and one in grasping the relationship with avoidance effect. Therefore, this study was aimed to find out how the negative images were delivered and recognized by subway users, and to specify whether or not the simultaneous stimulation of the negative emotional stimulus and auditory stimulus has an avoidance effect on the negative images. In the test, the negative images of IAPS and the classical music, which gives stability emotionally and psychologically, were presented at the same time, and by measuring sympathetic nervous system activity(LF/HF) as a psychological load index, heart rate variability(HRV) as RR triangular(an aggregate index), and the change in SCL(Skin Conductance Level) in galvanic skin response(GSR: Galvanic Skin Response) of automatic nervous system simultaneously, the traits were analyzed for how the avoidance effect affected the negative emotional stimulus. As a result of study, both HRV and GSR increased, showing a significant difference(p<0.05) before and after stimulation between Control(stable state) and Audio-Visual stimulus. This study result shows that there is no masking effect due to audio stimulus in an awakening state when we are exposed to the negative images. It suggests that as a method to increase the satisfaction in emotional response for subway users actually, the effective system needs to be established through the improvement and maintenance of subway advertisement system of the visual image, and that the factors for avoiding the media of negative images should be considered.