The current study aimed to analyze the types of conflict that pre-service teachers encounter during their practicum course, as well as a possible existence of relationship between features of practicum sites and the types of conflict. To achieve the goals, 130 pre-service teachers enrolling in department of early childhood four- and three-year universities located in Gyeongsangnam-do participated in a series of survey. The five types of conflicts included ‘self’, ‘lead teacher’, ‘children’, ‘institution’, and ‘peers’. The results showed that, first, ‘children’ conflicts showed the highest score. Second, the pre-service teachers of 3-years-old classes demonstrated the highest score in ‘conflict with children’. Third, the pre-service teachers of private daycare centers displayed the highest score in ‘conflict with the self’. Because not only do pre-service teachers acquire knowledge and skills needed for child care fields, but also they attain opportunities to move from theory to practice, practicum course is an essential procedure prior to becoming teachers. The findings of the present study implied the need for educational programs prior to conducting practicum in order to reduce conflict factors to the minimum.