This research was conducted to investigate the effects of a multicultural human rights education program on prejudice and multicultural attitude of elementary school students. For this purpose, a multicultural human rights education program was developed and applied to one fourth-grade class at an elementary school in Gwangju. The program was composed of eight themes. After the program, the mean score of prejudice decreased from 2.17 to 1.72, but the mean difference was not statistically significant. However, here were significant decreases in four subcategories of prejudice, namely culture, gender, ethnicity, and disability. The other subcategories of prejudice (i.e., family composition and appearance) showed no significant differences, despite a few qualitative changes observed. In multicultural attitude, the mean scores of the pre- and the posttest increased from 3.87 to 4.38 and the mean difference was statistically significant. Every subcategory of multicultural attitude such as openness, acceptability, and flexibility increased significantly, confirming positive effects of the education program on multicultural attitude. This study presents practical implications for developing multicultural human rights education programs in elementary schools and addresses challenges that we face in the transition from multicultural conflicts to equal society without discrimination.