The purpose of this study was to investigate the relative effects of parentification experience and emotional regulation on self-differentiation and psychological well-being in adolescents, and to probe for any the mediating effects of emotional regulation amongst the named variavles. A survey was conducted on 450 middle and high school students in the metropolitan area. Descriptive statistical analysis, Pearson correlation analysis, stepwise multiple regression analysis, simple regression analysis and multiple regression analysis using SPSS Win 21.0 were conducted for data analysis. The results of this study were as follows : first, self-differentiation was most defined by unfair and physical experieinces in parentification, follwed by an active style of emotional regulation. Second, psychological well-being was mostly accounted for by an active regulation style of emotional regulation, followed by an unfairness and emotional parentification experience. Third, an avoidant/distractive style of regulation style had a partial mediating effect in the relation between emotional parentification and self-differentiation. Finally, active, and support-seeking, style of reegulation completely mediated the relation between emotional parentification and psychological well-being.