The purpose of this study was to explore the process of changing learners perceptions and attitudes manifested through design thinking-based instructional experiences in pre-service teacher education. For this purpose, a total of 12 weeks of design thinking-based instruction activities were conducted for 14 students who took courses related to education at A University in Busan. For data collection and analysis, qualitative research methods based on grounded theory were applied, and semi-structured interviews, the learners’ personal and group reflective journals writing activities were conducted. The main results of this study are summarized as follows. First, based on design thinking learning was unfamiliar to most students as a first-time approach, but learning activities were facilitated and familiarized with learning through formative feedback of instructor and learning tutors with specifically provided forms. Second, based on design thinking team project environments are a major factor in the interaction process between peer learners, and this interaction process is based on the iterative process of converting and diverting peer learners’ opinions. In this process, opinions among the peer learners were not narrowed, so they had difficulty in selecting subjects, but most learners thought in a new direction through this process. observing a positive change in the back. Third, after the class, learners were able to look at their changed perception and attitude about design thinking through interviews, such as recognizing them as learning methods that foster cooperative learning skills. The main results of study can be suggest that various institutional and system-based changes are needed for design thinking environments, and provide directions for effective design thinking-based instruction in pre-service teacher education.