pH 7.4, $37^{circ}C$의 생리적 반응조건에서 단백질(bovine serum albumin, RNase A 및 lysozyme)을 glucose 또는 fructose와 반응시켰을 때, 단백질은 중합현상을 나타내었을 뿐만 아니라 아미노산 잔기도 크게 손상되었다. 이러한 현상들은 반응계로부터의 dicarbonyl 화합물의 생성과 관련이 깊은 것으로 관찰되어 dicarbonyl 화합물을 정량적으로 동정하였다. TLC와 mass spectrometry에 의하여 동정된 dicarbonyl 화합물로는 3-deoxyglucosone, glyoxal. methyl glyoxal 이었으며, 3-deoxyglucosone이 주된 생성물이었다.
When proteins (bovine serum albumin, RNase A and lysozyme) were reacted with glucose or fructose under physiological conditions of $37^{circ}C$ and pH 7.4, proteins were polymerized and also the amino acid residues of proteins were impaired. These phenomena were considered to be due to the formation of dicarbonyl compound from protein-sugar reaction systems, and therefore dicarbonyl compounds liberated were identified quantitatively by thin layer chromatography and mass spectrometry. 3-deoxyglucosone was the main product among dicarbonyl compounds.
