Optimal heat treatment process in martensitic stainless steel such as SUS416 is investigated. The approach is based on the combination of the interpolation and extrapolation method of a standard heat treatment technology with the principle of quenching and tempering temperature difference. The relationship of the macroscopic structure, fracture toughness and ductility as well as the hardness and strength are considered to induce a simple rule to apply with feasibility. Consequently, Optimal heat treatment condition in martensitic stainless steel is proposed and is shown the better quality. It was found that the smaller pain size of microstructure gives the enhanced fracture toughness and ductility.