The purpose of this research is to evaluate the improvements in the strength and injection range of loose ground after injecting $CaCO_3$(created by microorganism reaction). For this purpose, three cases of single-layer (Sand, SP, SW) specimens were made in a 150mm D ${ imes}$ 200mm H space and two cases of multi-layer specimens (SW/SP, SP/SW) were made in a 150mm D ${ imes}$ 300mm H space. The specimens were made with a relative density of 30% of soft ground and an injection was given over a time of one day. The uniaxial compression strength was measured with a cone penetrometer and the injection range was observed by checking the bulb formation around the injection nozzle. Also, the compositions of the specimens were assessed through XRD analyses. Based on the test results, a compressive strength of 500kPa and 15cm thick cementation were noted due to the cementation of the soil. This implies that there are significant effects of the pore condition and size on bio-grouting technology.