This paper describes the development of a specific ultra performance LC with electrospray ionization tandem mass spectrometric method for simultaneous determination of 13 quinolones in livestock and fishery products. The procedures involve the sample preparation based on solvent extraction without further clean up procedure. The method was validated according to the FDA guidelines. The limit of detection (LOD) and quantification (LOQ) were lower than 0.1 and $0.4{mu}g/kg$, respectively, and these were below the maximum residue limits (MRLs) established by European Union. Recoveries ranged from 87.5 to 104.7% for livestock products and 83.0 to 100.9% for fishery products. Relative standard deviations (RSD) ranged from 0.4 to 6.0% and 0.9 to 5.7%, respectively. The method was successfully applied to the analysis of quinolones residue. This quantitative method has many advantages including simple preparation, rapid determination, and high sensitivity, which could be applied to the determination of quinolones residues in foodstuff.