The purpose of this study was to examine the parental psychological control, mindfulness, and difficulties in emotion regulation on middle school students’ stress response. It also measured the mediating effect of mindfulness and difficulties in emotion regulation on young adolescent’s stress response. The participants were 308 middle school students attending middle schools in South Korea. Self-report questionnaires were developed to measure the students’ stress response, and they were administered along with parental psychological control-disrespect scale(PCDS), daily stress response inventory, the Korean version of child and adolescent mindfulness measure(K-CAMM) and the Korean version of the difficulties in emotion regulation scale(K-DERS). In order to verify the mediating effects, descriptive statistical analysis, correlation analysis, and a structural equations verification model were used through SPSS and AMOS, and the bootstrapping method was used to verify the statistical significant of mediating effects. The results of this study could be summarized as follows. First, parental psychological control, mindfulness, difficulties in emotion regulation and stress response had statistically significant correlations. Second, fromthe relationship between parental psychological control and young adolescents’ stress response, mindfulness and difficulties in emotion regulation were found to have sequential mediating effects. As the participants perceived that they possessed high psychological control, mindfulness decreased, and as mindfulness decreased, difficulties in emotion regulation increased, increasing stress response. Finally, the limitations if this study and future research directions are suggested.